|
|
|
|
|
| Martinez-paramo, Sonia; Horvath, Akos; Labbe, Catherine; Zhang, Tiantian; Robles, Vanesa; Herraez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R.; Cabrita, Elsa. |
| This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of... |
| Tipo: Text |
Palavras-chave: Cryobanking; Aquatic animals; Fish gametes; Germplasm. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00341/45172/44567.pdf |
| |
|
|
| Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc. |
| Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing... |
| Tipo: Text |
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf |
| |
|
|
|
